Mikael Kubista

Two-tailed PCR for Precision Diagnostics


Kubista is one of the pioneers in molecular diagnostics. He invented the light-up probes, which led to the foundation of LightUp Technologies AB as Europe´s first company focusing on qPCR based diagnostics. He co-foundedMultiD Analyses AB, which develops the market leading qPCR analysis software GenEx.In 2001 Kubista set up TATAA Biocenter as center of excellence in qPCR and gene expression analysis. TATAA Biocenter was the first laboratory in Europe to obtain flexible ISO 17025 accreditation and was presented the Frost & Sullivan Award for Customer Value Leadership as Best-in-Class Services for Analyzing Genetic Material in 2013. In 2014 TATAA introduced non-invasive prenatal testing (NIPT) in Sweden founding Life Genomics ABand in 2018 Kubista co-founded Gendoktorn, offering genetic testing and medical consultancy. Since 2007 Kubista also heads the Department of Gene Expression at the institute of Biotechnology of the Czech Academy of Sciences, developing methods for intracellular profiling.Kubista co-authored the MIQE guidelines and he is member of the CEN/ISO expert groups developing guidelines for the pre-analytical process in molecular diagnostics. Most recently Kubistas team invented Two-tailed PCR, which is currently the most sensitive and specific method for microRNA analysis.